Coding

Part:BBa_K3757003:Design

Designed by: Catia Harnack   Group: iGEM21_Tuebingen   (2021-09-25)


c_CHEN_1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A 6 His Tag was embedded into loop 5 and the AMP CHEN in loop 1. Includes Esp3I Typ IIS recognition sites.


Source

From Momordica cochinchinensis found in Cybase (http://www.cybase.org.au/index.php?page=card&table=protein&id=3) reverse transciped into dna sequence with codon usage optimized for Nicotiana bethaniana. CHEN sequenz was found on DBAASP ID: 9156 and was also reversed transcribed into dna sequence.

References